【摘要】 本研究应用牛乳头瘤病毒质粒(BPV-1)构建了一个表达载体pdBHhIL—4。该载体应用SV40启动子,可以有效地启动外源基因的表达,保留了BPV-1的氨基苄青霉素抗性(Amp~R)基因作为筛选标志,经限制性内切酶和Southern杂交分折提示构建是成功的,新的载体不仅促进了IL—4转化和表达,也为其它真核基因在真核细胞的表达提供了工具。更多还原

【Abstract】 An expression vector pdBHhIL-4 has been constructed using a bovine papilloma virus(BPV-l). In the vector, SV40 promoter has been introduced to efficiently initiate expression of foreign gene and Amp resistant gene of BPV-1 remained as a screen marker. Human interleukin 4 gene has been inserted into BamHI restriction endonuclease site which located at a downstream region of the promoter. The analysis by restriction endonuclease and Southern hybridization suggests that the construction is successful and the novel vector facilitates not only the transformation and expression of IL-4 but also other eukaryotic gene expressions in eukaryotic cells.更多还原