【摘要】 本文报告用抗人IgM(μ链)单克隆抗体ELISA法诊断血吸虫病。检测急性血吸虫病150例、慢性血吸虫病208例、经吡喹酮治疗后6个月粪检转阴的慢性血吸虫病28例、肝吸虫32例,阳性检出率分别为100.00％、88.46％、3.57％、15.63％;检测健康人50例均为阴性。动物实验早期诊断,观察3只感染血吸虫病猴,检测IgM(μ链)抗体,第28d均呈现阳性,比IgG-ELISA 42d、COPT 41d、粪检45d呈现阳性时间早。表明抗人IgM(μ链)单克隆抗体用于诊断血吸虫病,具有高度敏感性和特异性及疗效考核价值。更多还原

【Abstract】 By using monoclonal antibody of antihuman IgM (μ chain) with ELISA in diagnosis of schistosomiasis japonica was studied. The detecting sera were obtained from 150 cases of acute schistosomiasis. 208 chronic cases, 28 chronic cases with negative fecal examination 6 monthes after praziquentil therapy and 32 cases with clonorchiasis, the positive rate was 100%, 88. 46% 3. 57%and 15. 63%respectively . Test with sera from 50 healthy persons was all negative . Observation with sera from three monkeys infected with Schistosoma japonicum for experimental early stage diagnosis was found to be positive on the 28th day after infection. It was earlier than IgG-ELISA. COPT. and fecal examination which was positive on the day 42, 41 and 45 respectively. The results showed that this method had high sensitivity, sepcificity and could be of great value in checking the effect of treatment.更多还原