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血吸虫 疟疾 丝虫三联抗原片免疫酶染试验对三病监测的研究

PVC TRIPLE ANTIGEN SLIDE IEST: RESEARCH ON SEROLOGIC SURVEILLANCE IN AREAS WHERE SCHISTOSOMIASIS. MALARIA AND FILARIASIS ARE UNDER CONTROL

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【作者】 郑思民汤淡云何艳燕邱倩雯张月丽

【Author】 Zheng Simin, TangDanyun, He Yanyan, Qiu Qianwen, Zhang Yueli Shanghai Institute of Parasitic Disease Control and Research

【机构】 上海市寄生虫病防治研究所上海市寄生虫病防治研究所 上海 200335上海 200335上海 200335

【摘要】 PVC三联抗原片免疫酶染试验(IEST)系在PVC薄膜同一个三角形的凹井内,联合包被血吸虫尾蚴、恶性疟原虫和丝虫微丝蚴断片,用同一种免疫酶染技术以一滴血清同时检测3种寄生虫病抗体的检测方法。结果:血吸虫病环卵沉淀试验阳性者的检出率为95.74%,间日疟原虫血检阳性者的检出率为89.10%,历史丝虫微丝蚴阳性者的检出率为82.61%,99例健康者出现血吸虫阳性3例(3.03%),疟疾和丝虫阳性各1例(1.01%),经X~2检验,3种抗原各自对3病检测的特异性有非常显著意义(P<0.01)。 该法与3病常用血清学方法对1380例外来人口的监测比较和对4841例不同疫区人群的3病现场监测应用结果,均能反映各组人群的实际流行概貌。

【Abstract】 The antigenic material of PVC triple antigen slide, i. e. cercariae of Schistosoma jap. in vitro culture Malaria falciparum and sonicated microfilaria (B. malayi) are embeded in a membrane of PVC. The combined antigenic material is placed in a triangular well on the slide and IEST is carried out with the test serum. With a single drop of serum the test will give results for all the three diseases simultaneously. Thus it provides a simple and effective tool for the serologic surveillance of disease control in endemic areas.The IEST with the above antigenic preparation has been applied to migrant population and the results were consistant with the known incidence of the respective population tested.

【关键词】 PVC薄膜三联抗原片免疫酶染试验
【Key words】 PVe filmtriple amtigen slide. IEST
  • 【文献出处】 中国血吸虫病防治杂志 ,Chinese Journal of Schistosomiasis Control , 编辑部邮箱 ,1993年01期
  • 【被引频次】13
  • 【下载频次】25
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