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几种鸟类mt DNA限制性酶切图谱的比较研究

COMPARATIVE STUDY OF RESTRICTION MAPS OF SOME BIRDS

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【作者】 孙允明吴鹤龄

【Author】 Sun Yunming, Wu Heling (Peking University)

【机构】 北京大学生物系北京大学生物系 北京农业大学中心实验室

【摘要】 本文用5种限制性内切酶(Hind Ⅲ,BgLI,EcoRⅠ,PstⅠ和BamHⅠ)初步建立了狮头鹅、白鹅、山鸡、火鸡和鹌鹑的肝脏线粒体DNA的内切酶图谱。结果表明白鹅和狮头鹅的mtDNA的酶切图谱极为相似.山鸡、火鸡和鹌鹑的mtDNA限制性内切酶图谱之间的差异是很大的,以上事实说明驯化中的鹅类的不同品种间mtDNA差异不大。而鸡形目中的不同物种间的mtDNA是有很大差异性的。

【Abstract】 Five restriction cleavage maps of mtDNA from liver of Lionhead goose (Anser cygn-oides domestica), White goose (Anser cygnoides domestica), Pheasant (Phasianus colchi-cus), Turkey (Meleagris gallopavo) and Quail (Coturnix coturnix) are reported. Restriction endonucleases Hind Ⅲ, Pst I, BamH I, EcoR I and BgL I have 3, 3, 1, 3 and 4 sites from Phasianus colchicus, respectively. The restriction endonucleases have 6, 3, 3, 1 and 1 site from Meleagris gallopavo, respectively. The restriction endonucleases map of mtDNA from two variaties of Anser cygnoides domestica are the same, and these restriction enzymes have 5, 2, 2, 3 and 4 sites on those mtDNA, respectively. Restriction endonucleases Hind Ⅲ, Pst I, BamH I and BgL I have 3, 2, 1 and 1 sites from Coturnix colurnix.Analyzing five restriction maps, we discovered that.(1) There was high interspecific variation on fowl and goose;(2) There was no heterogeneity between two variaties of goose.

【关键词】 鸡类mtDNA鹅类mtDNA限制性酶切图谱
【Key words】 Fowl mtDNAGoose mtDNARestriction map
  • 【文献出处】 畜牧兽医学报 ,Chinese Journal of Animal and Veterinary Sciences , 编辑部邮箱 ,1993年06期
  • 【被引频次】15
  • 【下载频次】44
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