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谷子(Setaria italica)Ch4n×法氏狗尾草(S.faberii)杂种幼胚培养及杂种F1的鉴定

Immature Embryo Culture of a Cross between Setavia italica (Ch4n) and S.faberii and Studies on the Morphological and Cytological Characteristics of the F1 Plant

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【作者】 罗新谈郭扶兴周俊彦马惠吴权明朱光琴马云彤

【Author】 Luo Xin-tan;Guo Fu-xing;Zhou Junyan;Ma Hui;Wu Qun-ming;Zhu Guangqin;Ma Yun-tong Northwest Institue of Botary,Academia Sinica,Yangling Shaaxi 712100 Agricultural Academy of Shaanxi

【机构】 中国科学院西北植物研究所陕西省农业科学院粮食作物研究所陕西省农业科学院粮食作物研究所 陕西杨陵712100陕西杨陵712100

【摘要】 1989年,进行了谷子(Setaria italica)Ch4n×法氏狗尾草位(S.fabeiii)的远缘杂交和杂种胚培养。共接种已授粉子房264个,得到22株苗,仅11株成活。试管苗经继代培养和繁殖,得到了大量的小植株,移栽成活并发育到成熟。经形态学和细胞学鉴定,其中有两个株系是杂种,杂种F1的染色体数为2n=4x=36,花粉母细胞在减数分裂中期Ⅰ染色体配对不正常,染色体构型为(18-2)Ⅰ+(13-0)Ⅱ+(0-9)Ⅲ+(0-3)Ⅳ,后期染色体分离不正常,形成不正常的四分体和微型小孢子,花粉败育。F1高度不育,其形态特征介于双亲之间,植株在许多性状上都与父本法氏狗尾草相似,而与母本相差较远。

【Abstract】 Immature embryo culture of a wide cross, Setaria italica (Ch 4n)/S. faberii, was carried outin 1989. 22 seedlings were obtained from 264 pollinated ovaries cultured on modified MS me-dium with or without growth regulators, but only 11 survived after the second subculture.They were propagated into numerous plantlets and then transplanted to soil. Most of themgrew up to flowering. Results of cytological examination of the pollen mother cells at meiosisand observation of the morphological characters of the adult revealed that ouly 2 lines werebe hybrids whose chromosome numbers were 2n=4x=36, and whose chromosome pairing atthe metaphase Ⅰ of meiosis were irregular. The chromosome configuration was (2--18)Ⅰ+(0--13)Ⅱ=(0--9)Ⅲ+(0--3)Ⅳ and the chromosome disjunction at the anaphase Ⅰ of meiosis were alsoirregular. Abnormal tetrads and mini--spores were formed and the pollens were abortive. Thehybrid F1 plant was sterile and its morphological charactere were somewhat intermediate be-tween those of the parents, although nearer to the male parent.

【基金】 国家自然科学基金
  • 【文献出处】 作物学报 ,Acta Agronomica Sinica , 编辑部邮箱 ,1993年04期
  • 【被引频次】5
  • 【下载频次】69
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