【摘要】 为提高蚕豆的抗病性、抗虫性和着荚性,人们试图将其与野生近缘种进行种间杂交,但因杂交幼胚早期败育,至今未获成功。解决杂交幼胚的发育问题已成杂交成功的关键。作者以自交授粉15天的幼胚珠为材料,确立了由幼胚珠离体培养经继代直接成苗的方法,获得可正常开花结实的完整植珠。以蚕豆为母本与5个近缘种进行了种间杂交,对杂交20天的杂交幼胚珠进行了离体培养,经愈伤组织再分化形成试管苗。为克服蚕豆与近缘种杂交幼胚的败育进行了探索。更多还原

【Abstract】 In order to improve the resistance to disease, insect and increase the podding rate of faba bean (Vicia faba L.), crossing faba bean with its relative wild species is expected. But since abortion of hybrid young embryo occurs early, up to now there is not successful crossing. To overcome the difficulty in development of young embryo is thought as key problem. In this paper, a culture method which entire plantlet was obtained from young ovule was established by using ovule of 15 days after self-pollination. The plant obtained by using this method have normally bloomed and fructified. The interspecific crossing between faba(♀) and 5 relative species(♂)and in vitro culture of ovule of 20 days after pollination were carried out. A callus was formed and regeneration plantlet was obtained in tube. A valuable try was done for overcoming abortion of young embryo in crossing faba bean with its relative species.更多还原