【摘要】 从海产品中检出29株溶藻弧菌噬菌体,从中选出4株进行鉴定。据噬菌斑特征可分为两类:一类是透明,一类是不透明。但大小各有差异,直径均在0.5—3.0mm。电镜可见形态也可分成两种类型:即头部呈长轴六角形,尾部细长,结构简单;另一类头部呈等轴六角形,但棱角不甚明显,尾部很短。这些噬菌体的增殖效价均可达10^8-9 pfu/ml,对溶藻弧菌的综合裂解率为72.22%,单株平均裂解率在9.72—44.4%。4株噬菌体的特异性高,原液与612株属外常见菌作交叉裂解试验,均未出现交叉裂解现象;与697株属内弧菌测试,也仅对副溶血弧菌有39.0%的交叉裂解,但将原液稀释至10RTD 时,大部分交叉现象消失,表明两菌间有明显的亲缘关系。更多还原

【Abstract】 We identified 4 bacteriophages of V.alginolyticus in 29 ones,which were first isolated from seafood.According to their character of the plaques,they were classified into two kinds:one plaque was clear,the other was opaque.The size of these plaques were different and their diameters are 0.5-3.0mm.By electron microscopy observation,they could be classified into two kinds;one has a long axie and hexagemal head,and a thin-long tail,the other has an equal axie hexagenal head and a very short tail,but the edges and corners aren’t clear.The multiplication valence of the phages attained to 10^8-9pfu/ml.Total lysis rate of 4 bacteriophages was 72.22% to V.alginolyticus.However,the lysis rate of single phage was 9.72—44.4%.4 bacteriophages all had high host specificity.Cross-lysis reaction wasn’t found in the test of original solution of bacteriophages to 612 strains of different genus bacteria and 697 strains of genus Vibrio,but they only showed 39% cross- lysis rate to V.parahaemolyticus and most part of this phenomenon disappeared at 10 RTD of the phages.Thus,the obvious relation of consanguinity was showed between two kinds of bacteria strains.更多还原