【摘要】 <正> 生物素是一种小分子化合物,它以羧化酶辅酶形式微量存在于一切活细胞中,为生命不可缺少的物质。近年来,我们致力于微生物生物素高产菌株的筛选工作,对这一工作来说,一种快速简便且有效的生物素测定方法是至关重要的。已有的测定生物素方法很多,有化学法和微生物法。化学法易受更多还原

【Abstract】 A rapid auxanographic method for biotin determination was established by means of abiotin auxotrophic yeast strain,and a computer program was designed to calculate the data ob-tained.Its determination concentration ranges from 0.3 to 5.0 μg/ml.The time-tested routinework in our laboratory proved that it is rapid,precis and reliable.更多还原