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利用等电聚焦电泳研究黄单胞菌分类

A STUDY ON CLASSIFICATION OF XANTHOMONAS BY ISOELECTRIC FOCUSING

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【作者】 徐宝梁张志雍葛起新陈红燕

【Author】 Xu Baoliang Zhang Zhiyong Ge Qixing Chen Hongyan (Plent Quarantine Institute of Agricultural Minister,Beiiing 100026) Department of Plant Protection,Zhejiang Agriculltural University,Hangzhou.

【机构】 农业部植物检疫实验所浙江农业大学植保系

【摘要】 对黄单胞菌的4个种及29个致病变种的代表菌株进行了等电聚焦电泳研究,发现所测定的黄单胞菌种间、致病变种间蛋白质图谱差别很大。电泳结果经聚类分析表明,某些致病变种间的差别并不一定比种间的差别小,说明某些致病变种可以上升到种这一分类地位。引起细菌性黑颖病的黄单胞菌禾谷致病变种、小麦致病变种、大麦致病变种间差别很小,仅有6条蛋白质谱带的差别。因此这3个致病变种的分类地位需重新考虑,同时说明等电聚焦电泳对黄单胞菌种下分类具有一定指导意义。

【Abstract】 Studing Xanthomonas strains of 4 species and 29 pathovars by isoelectric focusing,itwas shown that the protein patterns were very different among the species and pathovars.Through cluster analysis of IEF date,it was found that the protein pattern differences among cer-tain pathovars were no fewer than that among the species.It revealed that some pathovarscould become to be species.There were few differences of 6 protein bands among X.campes-tris pv.cerealis,X.campestris pv.undulosa and X.campestris pv.hordei which cause bac-terial black cheff,so the classification of the three pathovars need to be re-considered.It alsoshowed that isoelectric focusing could be used to classify Xanthomonas below species.

  • 【文献出处】 微生物学报 ,Acta Microbiologica Sinica , 编辑部邮箱 ,1993年01期
  • 【被引频次】3
  • 【下载频次】39
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