【摘要】 对长梗木霉（Trichoderma Longibrachiatum）ANU₃-958纤维素酶的产生及提取进行了研究。结果表明,固体曲培养144小时,固体曲与浸提液比例为1:7（W/V）,采用硫酸铵分级沉淀法时所得纤维素酶活力最高。所得冻干纤维素酶粉经测定:羧甲基纤维素（CMC）酶活最高为2788.89IU/g,滤纸糖酶活（FPA）最高为79.44IU/g。相对固体曲得率平均为13.28%。更多还原

【Abstract】 The cellulase production and extraction from Trichoderma Longibrachiatum ANU₃-958 were reported. The results indicated that maximum enzyme activity can be obtained when the fungus was grown on a solid medium for 144 hours, the proportion between the solid culture and the extracting agent was about 1:7（W/V） and extract was precipitated twice by adding solid （NH₄）₂SO₄. Cellulase powder obtained by freeze drying was analysed for its related enzyme activities. The highest CMC and FPA enzyme activity are about 2788.89IU/g enzyme powder and 79.44IU/g enzyme powder respectively. The yield for the solid culture is about 13.28% averagely.更多还原