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FT3M、FT4M磁性固抗放射免疫分析方法学研究
RADIOIMMUNOASSAYS FOR FREE TRIIODOTHYRONINE AND FREE THYROXINE IN SERUM WITH MAGNETIC SEPARATION OF THE SOLID PHASE
【摘要】 FT3M、FT4M磁性固抗放射免疫分析法是采用特定的磁性固相抗体,集提取和分离为一体的一种灵敏而快速的直接二步法(反滴定法)。液体血清标准的适宜温育条件,用于直接测定人血清游离甲状腺激素(FT3、FT4)浓度。
【Abstract】 Two sensitive and rapid RIA method for quantitative measurement of free triiodothyronine (FT3) and free thyroxine (FT4) in serum are described. These two step RIA procedures utilize immunoextraction of free hormone and separation of free phase by specific, high affinity polyclonal antiserum coupled to cellulose coated magnetic iron oxide particles. The measurement range is 0—100 pmol/l for FT4 and 0—50 pmol/l for FT3 respectively. Sensitivity of FT3 and FT4 is no more than 0.5 pmol/l. The normal range is 2.5—9.5 pmol/l for FT3 and 9.5—25.5 pmol/l for FT4.
- 【文献出处】 同位素 ,Journal of Isotopes , 编辑部邮箱 ,1993年02期
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