【摘要】 为了解三苯氧氨（Tamoxifen）和雌二醇（E₂）对原癌基因c-myc表达的影响,在体外培养的兔子宫内膜细胞（M-60）,观察到给予E_2b小时后,原癌基因c-myc表达明显增加。E₂浓度在10^-10～10^-6mol/L范围内,c-myc的表达随E₂浓度加大而增强。Tamoxifen可拮抗E2所引起的c-myc的表达.当Tamoxifen的浓度为10^-5mol/L时抑制作用最明显,Tamoxifen的最佳抑制浓度是E₂浓度的10倍。本结果揭示在体外培养系统,E₂对子宫内膜细胞的增殖作用也可能是通过c-myc基因中介的。抑制c-myc基因的表达是Tamoxifen拮抗E₂刺激子宫内膜细胞增殖的原因之一。更多还原

【Abstract】 The influence of tamoxifen and estradiol on expression of c-myc protooncogenes of endometrial cell line（M-60） of rabbit was studied in the present paper. Endometrial cells were incubated in a modified Eagle’s medium by additional 6h incubation with estradiol or estradiol plus tamoxifen.The expression of c-myc gene was analysed by dot blotting. It was found that estradiol dose-dependently increased the expression of c-myc gene at concentrations of 10^-10～10^-5mol/L. The expression of c-myc gene induced by estradiol could be inhibited completely with 10^-5mol/L tamoxifen. These results suggested that the proliferation of the endometrial cell induced by estradiol in culture was mediated by c-myc gene. Inhibiting c-myc gene expression is one of the causes for tamoxifen in antagonizing the proliferative effect of estradiol on endometrial cells.更多还原