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低温保存的小鼠卵母细胞的体外授精研究

In Vitro Fertilization of the Cold-Stored Mouse Oocytes

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【作者】 李光鹏张心田

【Author】 Li Guangpeng Zhang Xintian(Department of Biotechnology, Northeast Agriculture College, Harbin)

【机构】 东北农学院生物工程系东北农学院生物工程系 哈尔滨 黑龙江省科学院自然资源研究所哈尔滨

【摘要】 采用75分钟和150分钟两种精卵作用时间,对经6℃低温处理2小时和6小时的小鼠卵母细胞进行体外受精。精卵作用75分钟后,经6℃处理的卵子无一受精,而对照组的受精率为30.7%。作用150分钟后,低温处理2小时、6小时和对照组的受精率分别为62.0%,36.55%和76.0%。并对试验所出现的现象作了讨论。

【Abstract】 White mouse oocytes were stored at 6℃ for 2 hours and 6 hours, and then tested their ability of fertilization in vitro. None of the stored oocytes was fertilized after 75 minutes of sperm-egg interaction. The rates of in vitro fertilization, however, were 62.0, 36.55, and 76.0% of 2 hour-stored, 6 hour-stored and the control oocytes, respectively after 150 minutes of sperm-egg interaction.

【关键词】 小鼠精子卵母细胞冷藏体外受精
【Key words】 MouseSpermatozoaOocytesCold-storedIn vitro fertilization
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