【摘要】 本文采用系列凝集素柱层析法,并配合外切糖苷酶处理研究了在视黄酸（RA）作用1—5天过程中人肝癌细胞株SMMC-7721细胞表面N糖链结构的变化。结果表明,RA促进3^H-甘露糖（Man）参入细胞表面N糖链,使高甘露糖型N糖链的百分比下降,复杂型百分比上升,并促进二天线N糖链的生物合成,使多天线特别是四天线和C₂,C₂₁b三天线N糖链的合成减少。结果提示,N糖链结构的这些变化可能是RA诱导SMMC-7721细胞向正常方向分化的结果。更多还原

【Abstract】 ³H-mannose labeled N-linked oligosaccharides were prepared from the cell surface of human hepatocarcinoma cell line SMMC-7721 treated with retinoic acid （RA） for 1—5 days and compared with untreated control cells by serial lectin affinity chromatography combined with exoglycosidase treatment. The results showed that RA stimulated incorporation of ³H-mannose into N-linked oligosaccharides on the cultured cell surface, decreased the percentage of high mannose type, and increased the percentage of complex type. Moreover, RA accelerated the biosynthesis of biantennary complex type, but decelerated the biosynthesis of tetraantennary and trianternnary, espically C₂,C_2,6 triantennary complex type N- linked oligosaccharides. It is suggested that the structural changes of N-linked oligosaccharides are associated with the Cell differentiation induced by retinoic acid.更多还原