【摘要】 本工作从自愿流产孕妇的胎儿取肝,按照LaBrecque法提取人肝刺激因子（human hepaticstimulator substance,hHSS）。用荧光探针Fura-2/AM测定离体肝细胞内游离钙,用离子分析仪测细胞染毒（四氯化碳CCl₄）前后基质中钾离子含量,观察hHSS对染毒肝细胞内Ca²⁺和K⁺稳态的影响,并测定肝细胞存活率和细胞内转氨酶（ALT）的漏出作为佐证。结果表明,人胎肝中含有hHSS,hHSS能提高离体肝细胞的存活率,维持肝细胞内游离钙的相对恒定,减少细胞内钾离子和ALT的漏出。这些结果提示,hHSS可保护肝细胞内钙,钾离子稳态和肝细胞膜的稳定,从而加强大鼠离体肝细胞抗CCl₄的损伤。更多还原

【Abstract】 The human hepatic stimulator substance （hHSS） was extracted from abortive fetalliver according to the method of LaBrecque. The intracellular free calciumion was mea-sured by fluorescent probes Fura--2/AM. Rat hepatocytes were isolated from liver bythe method of Seglen and intoxicated by CCl₄ vapour, then the changes of [Ca²⁺]_i, theleakage of intracellular K⁺, ALT and viability of hepatocytes were observed. The re-sults were as follows: The hHSS does exist in the human fetal liver, hHSS could increasethe viability of intoxiacated hepatocytes, maintain the intracellular calcium homeosta-sis, and decrease the leakage of intracellular potassium and ALT into the culture medium. These results indicate that hHSS could protect hepatocytes against CCl₄ throughmaintaining calcium homeostasis, preventing potassium leakage and sustaining stabilityof hepatocyte polasmalemma.更多还原