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稀土元素对仓鼠细胞和鸡胚细胞生长的影响

EFFECTS OF RARE-EARTH ELEMENTS ON THE GROWTH OF CHINESE HAMSTER AND CHICKEN-EMBRYO CELLS

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【作者】 潘中华陈海明陆克文闻孝来陈兵

【Author】 Pan Zhonghua~1 Chen Haimin~2 Lu Kewen~1 Wen Xiaolai~1 Chen Bin~1 (~1Shanghai Feed Quality Supervising and Testing Department,Shanghai 200030;~2Academy of Life Sciences,Fudan University,Shanghai 200433)

【机构】 上海市饲料质量监督检验站复旦大学生命科学院上海市饲料质量监督检验站 上海200030上海200433上海200030

【摘要】 为从细胞学水平揭示稀土元素对动物的促生长机理,本文选择中国仓鼠肺成纤维细胞(Wg3 1)和卵巢细胞(CHO),在含有不同浓度 STN 的1640完全培养液中培养;以及来自非免疫健康鸡群11日龄鸡胚所制成的鸡胚细胞,使之在0ppm、1.25ppm、2.5ppm、5ppm,10ppm、20ppm 和40ppm 稀土浓度的199培养液中培养.CHO 细胞的生长速率表现在10ppm浓度时细胞数最多,Wg3 1 和 CHO 的细胞分裂指数也以10ppm 时显著高于对照组(P<0.05).96小时鸡胚细胞单层面形成状况表明:三种类型的稀土化合物均以5ppm 浓度时对鸡胚细胞的促生长作用最明显,STV-2优于 CL-1,CL-1优于 CL-3.20ppm 和40ppm 浓度使 CHO、Wg3 1和鸡胚细胞生长受到抑制.结论认为,适量浓度的稀土可促进动物细胞生长,高浓度稀土抑制动物细胞生长.

【Abstract】 In order to reveal the mechanism in which rare-earth elements accelerate animal growth in the cytological level,Chinese hamster lung fibroblast (Wg31) cells and Chinese hamster ovarian (CHO) cells were cultured in the media-1640 with STN of different concentrations. One whole chicken-embryo hatched from eggs of healthy non-immunized chicks,was cul- tured in the media-199 containing rare-earth elements of 0 ppm,1.25ppm,2.5ppm,5ppm, 10ppm,20ppm and 40ppm respectively.CHO cell growth rate of 10ppm group was the high- est in five groups.And division index of CHO and Wg31 cells of this group was significantly higher than of the control (P<0.05).The development of the chicken-embryo cellular single-layer cultured for 96 hours indicated that all three kinds of rare-earth compounds ac- celerated cellular growth at 5ppm,STV-2 being superior to CL-1 and CL-1 to CL-3.How- ever,the growth of CHO,Wg31 and chicken-embryo cells were inbabited at 20ppm and 40ppm.The conclusion is that the proper concentration rare-earth elements can accelerate the growth of animal cells and high concentration rare-earth elements can inhabit the growth of animal cells.

  • 【分类号】S816·71;Q952.6
  • 【下载频次】37
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