【摘要】 用化学发光(CL)技术研究发现虫草煎剂(0.2～200 mg/L)和黄芪多糖(0.1～10mg/L)两种中药可促进人中性粒细胞(PMN)吞噬杀菌功能,并与各药物浓度呈剂量依赖关系.青霉素(1mmol/L)和氯丙嗪(0.08mmol/L)则分别促进或抑制 PMN 吞噬杀菌功能。用生物发光(BL)技术也证明虫草煎剂在一定浓度范围(0.2～200mg/L)内可增强 PMN 吞噬杀菌功能.并比较了 CL 法和 BL 法与 CFU 法,结果表明:用 CL 法和(或) BL 法代替 CFU 法研究药物对人 PMN 吞噬杀菌功能的影响是可行的.更多还原

【Abstract】 The results of experiment with chemiluminescence technique showed that both decoc- turn of fermented cordyceps sinensis sacc.(DFCSS)(0.2～200 mg/L)and astragalu polysac- charide(APS) (0.1～10 mg/L)copromoted bacteriocidal function and a dose-dependent rela- tion was evident with each agent.Increasion penicillin G(1 mmol/L)increased and chlopro- mazine(0.08 mmol/L)reduced bacteriocidal activities of PMN at therapeutic conceutration. Bioluminescence(BL)technique was also used in our experiment and results tested with this technique revealed that DFCSS within 0.2～200 mg/L was able to promote bactericidal ac- tivities of PMN.The results dectected by CL,BL and CFU methods were analysed and there a good coincidence with each method.Therefore,it is feasible to use CL and/or BL technique to substitute for CFU technique in the assessment of the effect of drugs on neutrophil phago- cytosis.更多还原