【摘要】 从橡胶树高产无性系(热研7—33—97)的新鲜胶乳制备总RNA,采用磁吸附法从总RNA纯化mRNA,加反转录酶得到cDNA,以cDNA为模板,设计并人工合成一对特异引物,采用聚合酶链式反应(PCR技术)扩增到一段约1.0kb的DNA片段,用1％低融点琼脂糖电泳回收目的DNA片段,然后将目的DNA克隆到pGEM—5Zf(-)质粒载体上。更多还原

【Abstract】 The total RNA was prepared from the fresh latex of Hevea trees (Reyan（7-33-97）. The mRNA was purified from the total RNA with the poly^ATtract^MT mRNA isolation system（Promega）, and added with AMV reverse transcriptase to synthesize cDNA. Two primers were designed and synthesised by using cDNA as a template, and a DNA fragments about 1.0kb was amplified with polymerase chain reaction （PCR）. Gel electrophoresis was performed on 1% low gelling temperature agarose, and the target DNA was then cloned into pGEM-SZf（-） plasmid using purified target DNA.更多还原