【摘要】 以人食管癌109细胞株免疫BALB/c小鼠获得两株抗食管癌杂交瘤,分别分泌单克隆抗体（McAb）2GF₃和2GF₄。以上述两种McAb的一种或将该二者混合,用ABC染色法检测食管癌和正常组织的石蜡切片,发现该两种McAb对不同组织类型或分化程度不同的食管癌细胞有不同的反应性;而且,应用两种McAb混合液的,可增高在食管癌总体标本中的检测阳性率。更多还原

【Abstract】 Using hybridomas resulted from fusions of mouse myeloma cells SP2/0 with spleen cells from BALB/c mice immunized with human E. Ca 109 cell line, following screening, two hybridomas 2GF₃ and 2GF₄, secreting respective monoclonal antibodies （McAb） against human esophageal cancer were obtained. By ABC staining, paraffin slice specimens from esophageal carcinoma patients were tested with either McAb 2GF₃ or 2GF₄, or their mixture.The rates of 2GF₃ antigen-positive reaction and those of 2GF4 were related with histological types and the degree of differentiation of esophageal carcinoma. The 2GF₃ showed higher positive reaction （89.4%） in high differentiated esophageal cancer specimens,while the 2GF₄ showed higher positive reaction （94.7%） in poorly differentiated ones. As a whole, positive rate with the 2GF3-2GF4 mixture was higher （83.6%） than that obtained with either 2GF₃（66.0%） or 2GF₄ （67.4%） and the intracellular granules of positive substance were bigger and thicker than those seen with either McAb.更多还原