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新疆犏牛胃肠道中G细胞的免疫组织化学定位及形态学研究

IMMUNOHISTOCHEMICAL LOCALIZATION AND MORPHOLOGICAL STUDY OF G CELLS IN THE GASTROINTESTINAL CANAL OF THE XINJIANG PIEN NIU

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【作者】 孙宝田; 吴亮生; 谢云芳; 郑丕芝; 朱庆华; 方之平; 潘黔生;

【Author】 Sun Bao-tian, Wu Liang seng Zheng Pi-zhi, Xie Yun-fang, Fang Zhi-ping~*, Pan Qian-sheng~(**) (Department of Histology and Embryology, Shihezi Medical College, Shihezi, Xinjiang;~* Department of Veterinary Medicine and Animal Husbandry,~(**) Department of Fishery, Huazhong Agricultural University)

【机构】 石河子医学院; 石河子医学院; 华中农业大学;

【摘要】 用ABC免疫组织化学方法对新疆犏牛胃肠道中G细胞进行鉴别,在胃和十二指肠部位均显示出阳性细胞。幽门处分布最密,向后则呈下降趋势,空肠至直肠须未见阳性反应。G细胞呈锥形、长梭形或不规则形。胞核呈空泡状阴性反应。胞质中颗粒明显,胞质突起常伸向腔面或邻近细胞或基膜。本文对新疆犏牛胃肠中G细胞的分布情况进行讨论,并和其它牛类和动物胃肠中G细胞作形态学方面比较。

【Abstract】 Using immunohistochemical staining technique of avidin-biotin-peroxidase complex (ABC) method, G cells were identified in the gastrointestinal canal of the Xinjiang pian niu [yak(♂) X cattle(♀) hybrid]. The positive cells were found in the gastric canal and duodenum,and the most density was in the pylorinc orifice, thereafter the distribution density was decreased gradually,and no positive reaction could be seen from the jejunum to the rectum. G cells were pyramid or long shuttle or irregular in shape. Nucleus showed negative reaction and empty caveola. In the cytoplasm,the hormone granules were clear,and the cytoplasmic processes often estended to glandular cavity or adjacent cells or basement membrane. In the present paper,the distribution of G cells in the gastrointestinal canal of Xinjiang pian niu was discussed.and the morphology of G cells of pian niu was compared with that of other cattle and animal.

【基金】 国家自然科学基金
  • 【被引频次】1
  • 【下载频次】19
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