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ELISA检测特异性IgE在囊虫病免疫诊断中的初步应用

Detecion of specific IgE in sera by ELISA and irsprllminary application immunodiagnosis of humancysticercosis

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【摘要】 应用酶联免疫吸附试验(ELISA)间接法检测了35例囊虫病患者血清中特异性IgE和特异性IgG,并将两者间的阳性率进行了比较。同时,对25例华支睾吸虫病患者和26例肺吸虫病患者的血清进行了交叉反应试验。结果:35例囊虫病患者血清中特异性IgE和特异性IgG的阳性率分别为88.57%和91.43%(P>0.05),而正常献血员、华支睾吸虫病和肺吸虫病患者的血清无一出现阳性反应。

【Abstract】 An indirect ELISA for the detection of specific IgE antibody to Cysticercus cellulosae in sera from the patients with cysticercosis was reported. 35 serum specimens from the patients with cysticercosis were detected. The results were compared with that of specific IgG. The (?)tive rate of specific IgE and IgG was 88. 57% and 91. 43% respectively (P>0. 05). The agreement rate of the two was 96. 88%. All serum specimens from healthy controls (35 cases), clonorhiasis (25 cases) and paragonimiasis (26 cases)were negative. The resuls show that the method is useful in the diagnosis of human cysticercosis.

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