【摘要】 本文报告用抗人载脂蛋白B（apo B）单克隆抗体（B₅）,检测不同个体等量低密度脂蛋白（LDL）的免疫反应性,发现其apo B均表现为高、中或低三种结合表型中的一种。遗传学调查结果证明,三种免疫反应表型变化系来源于apo B一对共显性等位基因（B₅⁺/B₅^-）的遗传,B₅^-基因频率为0.593,B₅^-为0.407。血脂测定结果表明,高胆固醇血症组的B₅⁺/B₅^-纯合子出现率明显低干正常组;在正常组内,B₅⁺/B₅⁺纯合子的血脂水平明显低于B₅^-/B₅^-纯合子,提示B₅⁺等位基因似与低血脂,特别是低胆固醇有关。更多还原

【Abstract】 A monoclonal antibody against human apolipoprotein B （apoB）,B₅,was used to test the immunnoreactivities of apo B in unrelated individuals. We found that the apo B in each person exhibited one of the three binding patterns: high,intermediate,or low affinity. Genetic investigation showed that these binnding patterns might result from co-dominant transmission of a pair of apo B alleles （B₅⁺/B₅^-）. The frequency of gene B₅⁺ was 0.593 and B₅^-was 0.407. We also found that the frequency of B₅⁺/B₅⁺ homozygotes in the hypercholesterolemic patient group was significantly lower than that in healthy subject group. The serum levels of total cholesterol, triglycerides, very low density lipoprotein （VLDL）—cholesterol, and VLDL—triglyeerides in B₅⁺/B₅⁺ homozygotes were significantly lower than those in B₅^-/B₅^-in the group of healthy subject. It is suggested that allele B₅⁺ might be associated with lower serum concentrations of lipids, especially the concentration of cholesterol.更多还原