【摘要】 本文针对苜蓿夜蛾Autographa californica核多角体病毒的多角体蛋白mRNA设计并合成了两个ribozyme。体外实验结果表明它们都能准确、高效地切割体外转录得到的mRNA片段。实验表明,在一定的范围内,切割百分率随着温度的升高、时间的延长和ribozyme浓度的提高而增加。Ca²⁺、Mn²⁺可以代替Mg²⁺作为ribozyme切割反应所必需的二价金属离子。更多还原

【Abstract】 Two ribozymes, ribozyme N-l and ribozyme N-2, were chemically synthesized, which are designed for blocking the expression of polyhedrin gene of Autographa californica nuclear poly-hedrosis virus (AcNPV). In vitro, they are able to specifically and effectively cleave the target mRNA fragments which were transcripted from part of polyhedrin gene. Results showed that the percentage of cleavage was increased when the temperature or time of incubation was increased or higher concentration of ribozyme was used. Ca2+, Mn2+ can substitute Mg2+ as the neces sary divalent cation for the cleavage reaction by ribozyme.更多还原