【摘要】 胆红素为人体内存在的胆色素之一,是细胞的代谢产物,对人体有重要的生理作用。检测人体血液中胆红素的含量,可作为诊断肝病的重要依据,在临床上有重要意义。但胆红素是一种热敏性和光敏性较强的物质,很不稳定,升高温度和光照都将加速其氧化,使检测结果偏低。为了确定极谱法检测胆红素的最佳操作条件,对胆红素在碱性水溶液中的稳定性进行了深入研究,试验了支持电解质的种类和浓度、溶液的酸度、溶液中的溶解氧、光照以及微量金属离子的存在对胆红素稳定性的影响。本文提出的极谱法测定胆红素的最佳操作条件为:0.2mol/LNH₃-NH₄Cl缓冲溶液,pH=9.5,试液通氮6min或加入4×10^-4mol/L EDTA溶液,避光,测定在0.5h内完成。更多还原

【Abstract】 Bilirubin is one of bile pigments existing within man’s body, and is the metabolic products of the cells. It plays an important role in physiology of man’s body. To examing the content of bilirubin in the blood of a body is of great significance in clinical practice and is an important basis to diagnose a liver diease. Bilirubin has higher sensitivities to heat and light. It will be oxygenated and lessened when it is heated or lighted. To define the best operation conditions that the content of bilirubin is determined with polarograghy, this paper studies the stability of bilirubin in basic aqueous solution, and takes experiments in the influences of battery electrolyte, pH, dissolved oxygen in solution, light and present of metalic ions. This paper puts forward the best operation conditions as 0.2 mol/L NH₃-NH₄C1 buffer solution, pH=9.5, solution is over flowed by introgen for 6 min or added by 4×10^-4 mol/L EDTA （ethylenedimine tetra acetic acid）, avoiding light, and the determination is completed in 0.5h.更多还原