【摘要】 重金属离子Cd²⁺、Hg²⁺和Pb²⁺均能替代Ca³⁺,与家兔阑尾B淋巴细胞中21kD钙结合蛋白(CaBP₂₁)结合,诱导其疏水区暴露,表现出相同的Phenyl-Sepharose疏水亲和层析特性;这些离子与Ca³⁺一样,不改变CaBP₂₁在SDS-聚丙烯酰胺凝胶电泳（PAGE）中的迁移;在重金属离子存在下,CaBP₂₁在非变性碱性甘油系统PAGE中的迁移较Ca²⁺存在时快,但比EGTA存在时慢,其迁移率的改变量与金属离子半径有关。更多还原

【Abstract】 Heavy me al cations including Cd2+, Hg2 + and Pb2 + can substitute for Ca2 + to bind CaBP21, a novel 21 kD calcium-binding protein purified from rabbit appendix B lymphocytes, and then to induce the exposion of it’s hydrophobic domain (s) .The migration of CaBP21 on SDS-polyacrylamide gel electrophoresis(PAGE)was the same in the presence of Ca2 + ,Cd2+Hg2 + ,Pb2 + and EGTA.On alkaline glycerol PAGE, CaBP21 migrated more slowly in the prosence of heavy metal cations then in the presence EGTA, but more quickly then in the presence Ca2+. The cation-induced changes in electrophofetic mobilities may be related to their ionic radii.更多还原