【摘要】 用CGE(N)衍生化甘氨酸,衍生化最佳条件为pH8;温度50±1℃,时间3～5小时。甘氨酸-CGE(N)的薄层荧光扫描峰面积与其量的回归相关系数均在0.99以上。检测下限为0.24μg(以甘氨酸计),线性范围在0.24～19.2μg之间,稳定时间为24小时。结果表明CGE(N)的可用性和方法的可靠性。更多还原

【Abstract】 A new stable fluoresentreagent, CGE （N）,was designed and syn-thesized in our laboratory. This paper pre-sents the results of using it to detect acompound having aminogroup. Glycine as an example was derived byusing CGE（N）, the best condition of deri-vatisation reaction was pH8, T 50±1℃,and time 3-5h. Spread agent was CHCl₃:CH₃OH: CH₃COOC₂H₅: iceHAc （1:3:5:1）.R_f of glycine-CGE （N） was 0.45, of whichfluorescent spectrum Em was 356nm （atEx=277nm）. The correlation coefficient between thefluorescent scanning area and quantitywas over 0.99, the linear relation retainedfor 24 h, the detection limit 0.24μg, andthe linear range 0.24-19.2μg.The data re-vealed that stability, sensitivity and re-producibility were good. These indicate that CGE（N） is a finefluorescent reagent for compounds havingprimary amino-group and this method isreliable.更多还原