节点文献

针对问号钩体赖型外膜的抗原分析

Antigen Analysis of McAb E4B7D5 Directed Against Outer Envelope of Leptospira interrogans Serovar lai by SDS-PAGE and Immunoblot

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 李亚非戴保民赵慧业

【Author】 Li Yafei;Dai Baomin;Zhao Huiye Research Unit of Leptospirosis Department of Pathophysiology

【机构】 华西医科大学钩端螺旋体病研究室华西医科大学病理生理学教研室

【摘要】 作者采用SDS-聚丙烯酰胺凝胶电泳和免疫印迹技术,对黄疸出血群赖型017株和601株、七日热群七日热型156株、澳洲群澳洲型620株以及塞马伦群帕托克型Patoc Ⅰ株等5株钩体外膜进行分析。检测到具有免疫保护作用和抑制钩体粘附作用的单克隆抗体E4B7D5能特异地识别赖型017株和601株钩体外膜的34.5kd和39.5kd两条蛋白带,而同其余3株钩体外膜蛋白无反应。由此提示该两条蛋白带可能为钩体外膜保护性抗原。

【Abstract】 McAb E4B7D5 was prepared by hybridoma technology in BALB/c mice immunized to outer envelope of Lep-tospira interrogans serovar lai. This McAbagglutinated rpecifically with all the 13serovars of Icterohaemorrhagiae serogroupin MAT test at high titres and protectedthe guinea pigs against the attack of viru-lent strain (017) of serovar lai. SDS-PAGEand immunoblot were used to analyse thereaction of the outer envelopes of the fivestrains of Leptospira (Leptospira interrogansIcterohaemorrhagic serogroup, serovar lai,017 and 601 stains; L.interrogans Hebdom-adis serogroup, hebdomadis serovar strain156; L. interragans Australis serogroup aus-tralis serovar strain 620; biflexa leptospirapatoc serovar strain Patoc I; with McAbE4B7D5. Results indicated that this McAbE4B7D5 recognized specifically 34.5kd and39.5kd outer envelope proteins of serovarlai, strain 017 and 601, so it might be animmunoprotective McAb directed againstLeptospira interrogans serovar lai. It wassuggested that 34.5kd and 39.5kd outerenvelope proteins of serovar, lai recognizedspecifically by McAb E4B7D5 might beimmunoprotective antigens.

【基金】 卫生部科研基金;国家自然科学基金~~
  • 【下载频次】5
节点文献中: 

本文链接的文献网络图示:

本文的引文网络