【摘要】 作者用反相离子对高效液相色谱法测定甘草及其制剂中甘草酸的含量。用ShimapckODS柱为分析柱,以水:甲醇=36:64(含0.005mol/L四丁基氢氧化铵)为流动相,用磷酸调pH至6.0,柱温35℃,检测波长254nm,最低检出限为13ng,平均回收率为99.85％。并研究了流动相中离子对试剂的浓度、甲醇含量、pH值及柱温等对容量因子和分离度的影响;考察了供试品前处理的方法。用本法测定了二种不同产地的甘草及十二种含甘草的中药制剂中甘草酸的含量,方法简便,有良好的重现性。更多还原

【Abstract】 Glycyrrhizin is the maincomponent of glycyrrhiza. In this articlean Ion-pair high performance liquid chro-matography(HPLC) method is described forthe determination of glycyrrhizin in twokinds of glycyrrhiza from different originsand twelve kinds of Chinese traditionalpatent medicine containing glycyrrhiza. Areversed phase system was used, includingan ODS column with water and me-thanol (36:64) as the mobile phase whichcontained 5mmol/L tetra-n-butyl ammo-nium hydroxide and was adjusted to pH 6.0by phosphoric acid. The column tempera-ture was 35℃; the detection was perform-ed at 254nm. Under this condition gly-cyrrhizin could be seperated from othercomponents. The limit of detection was13 ng, and the average recovery was99.85%. During the experiments, we studiedthe concentration of counter-ion, the mix-ing ratio of methanol to water, the pH ofthe mobile phase and column temperatureand their effect on the capacity factor ofcompound and resolution. We also studiedthe method of pre-treatment for the sam-ples. The determination method is simpleand accurate.更多还原