【摘要】 采用体外细胞培养,用生物倒置显微镜观察,发现牙周病优势菌群之内毒素脂多糖(LPS)使人牙龈成纤维细胞(HGF)形态发生不同程度的变性改变,甚至死亡自溶。透射电镜观察发现,LPS使HGF的核、线粒体、高尔基复合体、内质网、溶酶体等多种细胞器形态异常。不同种类的细菌的LPS,使HGF超微结构改变基本一致。LPS 可能是作用于线粒体而发挥其毒性效应的。更多还原

【Abstract】 Using the techniques of cell culture in vitro,biological inverted microscope and transmit electronic microscope,the effects of periodontal bacterial LPSs on the morphology and ultrastructure of HGF were investigated.The re-suits showed the degenerations of LPSs affected HGF to various degrees and necrolysis of cells in the severe cases.EM examination revealed the diffuse morphologic alterations of HGF involving many cellular organelles caused byLPSs,including the nuctin,the mitochondria,the Golgi complexes,the endoplasmic reticuta and the lysosomes.Thetoxic machanism of LPSs may rely on their effects on the mitochondria.更多还原