【摘要】 本文采用Schnaitman处理法提取了鼠伤寒杆菌的外膜蛋白（OMP）。用提取的OMP免疫家兔和小鼠,经ELISA方法测定家兔、小鼠及鼠伤寒感染患者血清中抗-OMP抗体效价,并用小鼠作足垫肿胀实验和主动及血清被动免疫保护实验。结果免疫动物和病人血清中都含有较高滴度抗体,免疫小鼠足垫出现明显的DTH反应。50μg OMP免疫小鼠可保护500 LD₅₀毒株攻击,0.2ml免疫血清也能够被动保护以上同样量的毒株攻击。这些结果表明,实验提取的OMP有较强的免疫原性和明显的免疫保护作用。应用Western blot分析兔、鼠及病人免疫血清均能识别36KD蛋白带,36KD蛋白带可能是鼠伤寒杆菌的主要免疫原。更多还原

【Abstract】 OMP of S. typhimunrium were extracted with the method of described by Schanitman. Rabbits and mice were immunized with the extracted OMP. The serum antibody titer of rabbits,mice and patients with typhimurium was measured, and footpad swelling experiment be done with mice. Mice were used as active and passive experiment. The result showed that both rabbits and mice had high antibody titer in serum. Patients infected by S. typhimurium also had high antibody titer in serum. Immunized mice pad exhibited obvious DTH response. Mice immunized with 50μg of OMP could be protected from the attack by 500 LD₅₀ of wild strain of S. typhimurium,and 0. 2ml immune serum also could passive protected mice to attacked by above same 500 LD₅₀. This result indicated that the extracted OMP had strong immunogenicity and obvious immune protection. Immune serum of OMP was analyed with western blot. The result showed that 36KD OMP band could be identified by all immune serum of OMP. The 36KD proteins（porin） may be a major immunogen of S. typhimurium.更多还原