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肺癌患者与正常人血清蛋白SDS电泳银染的比较分析

ANALYSIS ON THE SERUM PROTEINS OF LUNG CARCINOMA PATIENTS COMPARED WITH NORMAL PERSONS USING SDS GEL ELECTROPHORESIS SILVER NITRATE CHROMATIC STAIN WAY

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【作者】 孙思全叶合生朱泽瑞

【Author】 Sun Siquan;Ye Heshen Hunan Medical Junier College Zhu Zherui Hunan Biology Research Institute

【机构】 湖南省医学专科学校湖南省医学专科学校湖南师大生物系生物研究所

【摘要】 本文采用聚丙烯酰胺凝胶电泳硝酸银染色法,对41例肺癌患者和41例正常人的血清蛋白(简称SP)进行了比较分析,发现肺癌患者与正常人的SP电泳图谱有明显的差别.经扫描后统计检查,发现谱带的数目变异以及相对百分含量的差异均达到极显著水平(t=4.4955)及:t=9.5300).A区和F区以及B区和G区郡具有明显的特征谱带.这些特征谱带有可能用于早期诊断肺癌;因而本实验方法可望成为肺癌普查的筛选方法之一.

【Abstract】 In this experiment,the serum protein(SP)of fourtyone lung carcinoma patients were compared with fourtyone persons with SDS gel electrophoresis silver nitrate chromatic stain way.It was discovered that patient’s electrophoresis pictures of SP differ from normal persons.The difference have arrived at extremely evident level in the number and content of isozymes(t=4.4955**and t=9.5300**).There were abvious characteristic bands in the A.F and B.G district.Therefore,it was possible these charaxteristic band be used of medicine as careinemie diagnosis.It was hopeful that the way be emplyed to investigate carcinoma.

【关键词】 肺癌血清蛋白SDS凝胶电泳
【Key words】 carcinomaserum proteinSDS gel electrophoresis
  • 【文献出处】 湖南师范大学自然科学学报 ,Journal of Natural Science of Hunan Normal University , 编辑部邮箱 ,1993年01期
  • 【分类号】R734.2
  • 【下载频次】71
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