【摘要】 探讨了α-甲基-1-酪氨酸(α-methyl-1-tyrosine简称AMT)的放射性碘标记、分离纯化方法及其在小白鼠体内的分布。结果显示:采用氯胺-T法或Iodogen法标记,其标记率最低为60％,最高达80％以上。采用Sephadex LH-20柱层析分离~*I-AMT,其放化纯度均大于98％。~*I-AMT从血中清除迅速,主要经肾脏排泄;静注给药后5、15、30、60min,每克脑组织分别占注射剂量的4.14％、2.50％、2.13％和1.24％。更多还原

【Abstract】 Radioiodinated AMT is a new positive imaging agent for brain tumour. In this paper, its radioiodination, purification, and biodistribution in mice is described. AMT was labelled with 125I/131I using either chloramine-T or iodogen method. The labelling rate was 60% to 80%. After column chromatography on sephadex LH-20, the radiochemical purity was greater than 98%. Animal experiments showed that 25I-AMT was eliminated from blood rapidly and was mainly excreted through kidney. 5, 15, 30, 60 minutes after tail vein injection, the radioactivities of brain tissue per gram was 4.14%, 2.50%, 2.13%, 1.24% of injection dose respectively.更多还原