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硫酸盐还原菌还原铬(Ⅵ)的研究

Study on Removing Hexavalent Chromium by Sulfate-Reducing Bacteria (SRIV).

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【作者】 汪频李福德刘大江

【Author】 Wang Pin etal. ( Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu)

【机构】 中国科学院成都生物研究所中国科学院成都生物研究所 成都 610041成都 610041成都 610041

【摘要】 从电镀厂废水的淤泥中,用Hungate厌氧技术分离出一株高效还原铬(Ⅵ)的硫酸盐还原菌,菌体为弧形杆状,宽0.5—0.8gμm,长2—4.2μm,革兰氏阴性,极生单鞭毛,作无规则的渐近式运动,无芽孢,无荚膜,不完全氧化乳酸盐到乙酸盐,能利用可溶性淀粉、葡萄糖、蔗糖、H2+CO2、乙醇、甲酸盐等为唯一碳源。经鉴定属于Desulfovibrio sp.(SRIV)。用SRIV菌进行去除铬(Ⅵ)的实验表明最适生长温度30℃,最适pH值7,耐受铬(Ⅵ)的最高浓度10×10-3mol/L,还原铬(Ⅵ)最适量4×10-3mol/L。对铬(Ⅵ)去除率可达99.8%。SRIV菌生长范围广,性能稳定,因此,SRIV菌可作为净化含铬废水的高效功能菌。

【Abstract】 A strain of sulfate- reducing bacteria capable of reducing hexavalent chromium was isolated from electroplating sludge by using Hungate anaerobic technique. The strain was a motile curved rod, possessing singly polar flagella. The cell was 0. 5-0. 8μm in length, non- sporing, non- capsules, Gram-negative. The oxidation was incomplete, leading to acetate production; and glucose, sucrose, pyruvate, H2 + CO2, alcohol, and formate were fermented in the absence of an external electron acceptor. These characteristics established the isolate as Desulfovibrio sp. Reclaiming of hexevalent chromium with the isolate has shown that it was resistant to Cr(VI) concentrations of up to 10 ×10-3mol/L at the optimum temperature 30℃ and at optimum pH 7. 0. And under such conditions, the optimun Cr(VI) concentration for its growth was 4×10-3mol/L. The removal of Cr(VI) was 99. 8 %. SRIV strain was a engineered strain in removing Cr(VI) from wastewater.

【基金】 本课题为国家自然科学基金项目
  • 【文献出处】 环境科学 ,Environmental Science , 编辑部邮箱 ,1993年06期
  • 【被引频次】76
  • 【下载频次】369
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