【摘要】 豌豆种子在蒸溜水中预浸24h 后,放入0.000,0.002、0.020、0.200、2.000 m molL^-1叠氮化钠溶液处理24h,研究叠氮化钠对豌豆的诱变效应。试验所用的测定指标包括 M₁代的发芽率、田间出苗率、萌发延滞时间、根长、染色体畸变率、过氧化物酶、淀粉酶和蔗糖转化酶活性及 M₂代叶绿素缺失突变率.试验结果表明:（1）高浓度叠氮化钠溶液(≥0.020mmolL^-1)降低种子萌发率和田间出苗率,抑制根的生长,延滞种子萌发,抑制酶活性随萌发过程的增高;（2）低浓度叠氮化钠溶液(0.020mmol L^-1)处理刺激根的生长和酶活性的增高;（3）叠氮化钠有效诱发 M₂代叶绿素缺失突变。所有突变体中,黄绿苗突变体发生频率最高.0.200 mmol L^-1叠氮化钠溶液处理,叶绿素缺失突变频率高达50.53%;（4）叠氮化钠不诱发根尖染色体畸变。更多还原

【Abstract】 Seeds of pea（Pisum salivum L） were presoaked in distilled water for 24h and treated with 0、0.002、0.020、0.200、2.000 m mol L^-1 sodium azide solutins to study mutagenic effects of sodium azide in pea.Criteria used in the experiment included germination、field seedling、root length、chromosomal aberration frequency,activities of peroxidase、amylase and in- vertase in M₁ generation as well as chlorophyll—deficient mutation frequency in M₂ germina- tion.The experimental results showed:（1）the sodium azide treatments of higher concentration(≥ 0.020 m mol L^-1) decreased germination and field seedling,inhibited root growth,delayed germi- nation and inhibited the increases of enzymes along with germination course;（2）the sodium azide treatment of lower concentration(0.0020 m mol L^-1) stimulated rooth growth and the increases of enzymes;（3）sodium azide proved effective for inducing chlorophyll deficient mutations in M₂ generation.Among all chlorophyll—deficient mutents,the frequency xantha—viridis was the high- est.The frequency of chlorophyll—deficient mutations induced with 0.200 m mol L^-1 azide solu- tion was as high as 50.53%;（4）sodium azide did not induce any chromosomal aberration of root tip.更多还原