【摘要】 球孢白僵菌017突变菌株（Beauveria bassiana 017）在CTN培养基（含0.2～1％壳聚糖,0.075％KH₂PO₄,0.05％MgSO₄,0.05％NaCl,0.001％FeSO₄,0.3％KNO₃,0,1％牛肉膏,pH5.0）中,20～30℃,200r·p·m振荡培养7天,可以分泌大量的胞外壳聚糖酶,对底物水解的最适温度为45～50℃,最适pH值为3.8～4.0。K_m值和V_max分别为2.17×10^-6M和0.45μmol/min,60℃保温1小时,剩余酶活性为10％,Ca²⁺,Mn²⁺和Mg²⁺对酶有激活作用,Cu²⁺和Fe³⁺对酶有部分抑制作用,Hg²⁺和Ag⁺则强烈抑制酶的活性。更多还原

【Abstract】 Beauveria bassiana 017 incubated in CTN medium (contained 0.2-1 % Chitosan, 0.075% KH₂PO₄, 0.05% MgSO₄, 0.05%NaCl, 0.001%FeSO₄, 0.3 % KNO₃ and 0.1% beef extract, pH 5.0 at 20-30℃ and 200rpm for seven days can secret Chitosanase quantitatively. The optimum pH and temperature,the Km value and Vmax for Chitosanase were 3.8-4.0, 45-50℃, 2.17× 10^-6M and 0.45μmol/min,repectively. Chitosanase lost 90% of its activity at 60℃ for 1 hour. This enzyme was activated by Mn²⁺,Mg²⁺ or Ca²⁺,while strongly inhibited by Hg²⁺ or Ag⁺,and partly inhibited by Cu²⁺ or Fe³⁺.更多还原