【摘要】 本实验用12株经鉴定及增菌培养的幽门螺杆菌(Helicobacter Pylori;HP)制成酸提取物作抗原,以ELISA间接法对101名患者进行血清HP-IgG检查,同时做细菌学检查,并以此作为金标准。检查结果显示62例患者血清HP-IgG阳性,阳性率达61.39%,与细菌学检查相比较,ELISA间接法检测HP感染的敏感性和特异性分别为:93.55%、89.74%。由此我们认为ELISA间接法检测HP抗体可用于HP感染的流行病学调查及大量标本的初筛检查。更多还原

【Abstract】 In this experiment,by using the ELISA indirect method, we detected serum HP-IgG in 101 patients with acid extractable antigens which were prepared from twelve HP strains by appraising and breeding cultivation. Detection of bacteriology was made at the same time as a gold standard. @The results were:serum HP-IgG of 62 patients was positive, comparing with the bacteriological examination, the sensitivity of ELISA indirect method was 93.55%. The specificity was 89.74%. @From the above results, we considered that detection of HP antibody, by using ELISA indirect method,could be used in the epidemiological investigation of HP infection and in the preliminary detection of a large number of samples.更多还原