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大鼠肝与肺及人胎肝与肺微粒体酶对苯并(a)芘代谢的研究
Metabolism of Benzo(a)pyrene by Rat and Human Liver and Lung Microsomes
【摘要】 苯并(a)芘[B(a)P]是一种致癌性多环芳烃,本文对大鼠肝、肺及人胎肝、肺微粒体酶在体外对B(a)P的代谢进行了研究,通过差速离心法获得的微粒体酶与B(a)P在37℃中温育1h。经有机溶剂提取的代谢产物用反相高压液相分析,结果表明各种组织中均有六种代谢产物可被检测到。其中3种为二氢二醇苯并(a)芘(9,10-diol-B aP;7,8-diol-B aP;4,5-diol-Ba P),2种羟基苯并(a)芘(9-OH-B aP;3-OH-B aP)以及1种醌基苯并(a)芘(quinone-B aP)。在二氢二醇苯并(a)芘中,7,8-diol-B aP易于被激活成能与DNA形成共价结合的环氧化物苯并(a)芘,说明除肝脏外,人肺组织也能直接代谢激活苯并(a)芘。
【Abstract】 Benzo (a)pyrene [B(a)P]is a kind of widespread carcinogenic aromatic polycylic hydrocarbon which must be metabolized by mixed function oxidase (MFO) to form ultimate carcinogen. MFO is different not only between the races but also the organs. For this reason,B(a)P metabolism in vitro by microsomes extracted from rat liver, rat lung, human fetal liver and human fetal lung was studied. Microsomes obtained through differential centrifugation was incubated with B(a)P in metabolic mixture at 37℃ for one hour. The metabolites was analysed by reversed-phase HPLC. The results show that the three kinds of dihydrodiol-BaP (9,10-diol-BaP; 4,5-diol-BaP; 7,8-diol-BaP), two kinds of hydroxy-BaP (9-OH-BaP; 3-OH-BaP) and one kind quinone-BaP can be detected. Among them, 7, 8-diol-BaP is more likely to be activated to form BaP-diolepoxide which can covalently bind to DNA. It means B(a)P can be metabolized not only by human liver,but also by human lung.
- 【文献出处】 广州医学院学报 ,Academic Journal of Guangzhou Medical College , 编辑部邮箱 ,1993年01期
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