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氢化物-原子荧光法测定红细胞膜结合硒的研究

HYDRIDE GENERATION-ATOMIC FLUORESCENCE SPECTROMETRY DETERMINATION OF BINDING SELENIUM IN HUMAN ERYTHROCYTE MEMBRANE

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【作者】 杨根元徐德选邢光伟杨大惠

【Author】 YANG Genyuan and XU Dexuan Department of Instrumental Analysis, Zhenjiang Medical College, 212001 Zhenjiang YANG Dahui Zhenjiang Enviromental Monitoring Centre, Zhenjiang

【机构】 江苏镇江医学院仪器分析教研室镇江市环境检测中心站 212001 镇江212001 镇江212001 镇江

【摘要】 本文用氢化物-原子荧光法测定红细胞膜结合硒含量。测定健康者30人,膜结合硒平均值为12.50±0.65ng/mg膜蛋白;原发性高血压患者26人,膜结合硒平均值为8.58±0.42ng/mg膜蛋白;冠心病患者30人,膜结合硒平均值为8.32±0.34ng/mg膜蛋白。测定结果表明,原发性高血压组和冠心病组胞膜结合硒含量明显低于对照组(P<0.01)。该方法的回收率为96.23~100.70%,相对标准偏差小于4.80%。

【Abstract】 The present study was performed to determine the amount o(?) human erythroeyte membrane by using hydride generation-atomic fluorescence spectrometry. In thirty healthy subjects, the average of binding selenium in erythrocyte membrane was 12.5±0.65ng/mg membrane proteins. In twenty-six patients with primary hypertensin, the average of binding selenium in erythrocyte membrane was 8.58±0.42ng/mg membrane proteins. In thirty cases with coronary heart disease, the average of binding selenium in erythrocyte membrane was 8.32±0.34ng/mg membrane proteins. The difference was significant (P<0.01). As to the determination method, the rate of recovery varied from 96.23% to 100.70% and the RSD was less than 4.80%.

  • 【文献出处】 光谱学与光谱分析 ,Spectroscopy and Spectral Analysis , 编辑部邮箱 ,1993年02期
  • 【被引频次】13
  • 【下载频次】49
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