【摘要】 设计一对与pUC-18质粒多克隆位点两端顺序互补的引物(22bp)。用PCR扩增的方法,可以制备DNA克隆片段。如在PCR扩增时掺入α-(32)~P-dATP,可将插入片段进行同位素标记作为探针直接用于Southern印迹杂交。此法亦可用于临床基因诊断。更多还原

【Abstract】 A pair of PCR primers were selected from the sequence flanking cloning site of pUC18, and an universal PCR procedure was constructed for the preparation and radiolabelling of DNA inserts cloned in pUC plasmid. This PCR procedure facilitated the identification of insert DNA length as well as preparation of inserts. The effectiveness of radiolabelling by PCR was identified through Southern blotting of some human X chromosome specific single copy probes.更多还原