【摘要】 采用快速Fmoc合成技术,在多抗原肽(MAP)树脂上直接合成了带有8拷贝HCV核壳蛋白区免疫原性肽段C-19的MAP-C-19分支状大分子,研究表明,MAP-C-19分支状合成肽抗原检测HCV血清抗体的检测灵敏度有所提高,已达到HCV诊断试剂国家质控标准的要求。更多还原

【Abstract】 A branched biomacromolecule MAP-C-19, which contains eight copies of immunogenic peptide C-19 from the nucleocapsid protein of HCV, was directly synthesized on the eight-branch MAP resin with the Fast Moc technique. As shown by reverse-phase HPLC and protein sequencing, the synthetic branched peptied was both pure and chemically defined. Indirect ELISA with the MAP-C-19 peptide as coating antigen was used to test 47 serum samples in addition to the quality-control panels for sera prepared by ourselves or supplied by the National Institute for the Control of Pharmaceutical and Biological Products. The detection sensitivity was found to be higher than unbranched C-19 peptide and up to the nationalstandard.更多还原