【摘要】 应用地高辛配体(digoxigenin)标记探针的原位杂交技术,检测了原癌基因 c-myc 和 jun在12例不同发育阶段的人胎心肌中的表达。根据胎龄,将其中9例分成三组,胎龄分别是16—17、23—24和27—28周,每组各3例。另外有32和36周及足月的大胎龄标本各例。杂交信号用图象分析仪(MIAS 300)分析处理。结果显示:c-myc mRNA 或 jun mRNA 的表达水平在3组心肌样本中的任意2组间的变化有差异显著性(P<0.01)结果表明,在胎儿早期,c-myc 和 jun 的表达都较高,随着胎龄的增加,其表达水平逐渐下降,在足月胎儿表达水平很低。结果提示,在人胎心肌的发育过程中,这两种原癌基因的表达均是一种下行调节方式,反应了 c-myc 和 jun 在心肌细胞的发育过程中与心肌细胞的生长密切相关。可能在心肌细胞的增殖与分化中起着重要调控作用。本研究也表明,地高辛配体标记探针的原位杂交技术具有灵敏度高,分辨力好和实验周期短等优点。对在体正常心肌发育的原癌基因表达的研究,有助于对正常心肌生长以及对心肌肥大发生的分子机制的了解。更多还原

【Abstract】 By means of in situ hybridization with digoxigenin as a probe label,we detected expres- sion of proto-oneogenes,c-myc and jun,in 12 cases of myocardium of human fetus during embryonic age.According to the embryonic age,9 different casess were divided into three groups,weeks 16-17,23-24 and 27-28 respectively.Each group was composed of 3 cases.In addititon,there were 3 cases of late embryonic ages,weeks 32,36 and full term.one for each.Hybridized signals were analyzed with a computer-assisted image-analyzing system (MEAS 300).A statistic analysis demonstrated that there was significance of defference be- tween the mRNA levels of myocardium of any two of 3 groups(P<0.01)either for proto- oncogene c-myc or jun.The present study showed that in the early stage of human fetus, both c-myc and jun were expressed at higher level,their expression was decreased when em- bryonic age increased and was very low in a full term fetus,The results suggested that dur- ing developmental stages of human fetus,the pattern of expression of both c-myc and jun is developmentally ndown-vegulated.Such a pattern implied that paroto-oncogenes c-myc and jun are closely related to myocardial growth,and that they may play an important role in regulating proliferation and differentiation of myoardium during development.Furthermore, the study of expression of cellular oncogenes in normal myocardium in vivo is helpful not on- ly for understanding the normal myocardial growht,but also for eluciating the molecular mechanism of heart hypertrophy.更多还原