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大鼠垂体远侧部内分泌细胞的四重免疫组织化学染色方法
A QUADRUPLE IMMUNOHISTOCHEMICAL STAINING METHOD FOR THE ENDOCRINE CELLS IN THE PARS DISTALIS OF RAT PITUITARY GLAND
【摘要】 本文介绍一种显示大鼠垂体远侧部内分泌细胞的四重免疫组织化学染色方法。此法以PAP 法为基础,结合 DAB 银加强法和间接免疫金标记技术。方法比较简单,所需试剂容易得到。
【Abstract】 A new multiple immunohistochemical staining method was introduced which could be used to show four hormons simultaneously in a single tissue section of rat pituitary pars dis- tails.The new method was based on Sternberger’s PAP method which was combined with silvetr intensified DAB chromogen technique and indirect immuno-gold labelling technique. The method was simple,and the required reagents were easy to acquire.
【关键词】 多重免疫组织化学染色法;
垂体远侧部;
【Key words】 Multiple immunohistochemical staining method; Pars distalis of pituitary gland;
【Key words】 Multiple immunohistochemical staining method; Pars distalis of pituitary gland;
- 【文献出处】 中国组织化学与细胞化学杂志 ,Chinese Journal of Histochemistry and Cytochemistry , 编辑部邮箱 ,1993年04期
- 【被引频次】1
- 【下载频次】35