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胃粘膜病变核仁组成区嗜银蛋白的银染计量和能谱分析

SILVER STAINING MEASURE AND ENERGY SPECTRUM ANALYSIS ON ARGYOPHILIC PROTEIN IN THE NUCLEOLAR ORGANIZER REGIONS OF GASTRIC MUCOSAL LESION

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【作者】 赵振金徐根兴余有训喻方迩陈进贵张丽张运芳

【Author】 Zhao Zhenjin Xu Genxing Naval Medical College Nanjing,210049,P.R.China

【机构】 南京海军医学专科学校江苏省人民医院南京马群医院 南京 210049南京 210049

【摘要】 对77例胃粘膜活检标本进行银染,显示核仁组成区相关的嗜银蛋白(Ag-NOR),进行计数和统计分析,并对银染标本作电镜观察及能谱分析。结果表明,Ag-NOR 颗粒计数的多少,对胃肿瘤恶性程度的诊断有重要参考价值。电镜证实银染物质定位于核仁组成区,银染阳性区能谱分析显示 Ag 峰,说明银染反应具特异性.

【Abstract】 A quantitative and statistical analysis has been made on the relative at- gyrophilic protein in the nucleolar organizer regions (NOR) of gastric mucosalbiopsy specimens of 77 cases by using the silver staining method.An electronmicroscopic observation and an energy spectrum analysis have also been madeon the silver stained specimen.The results showed that Ag-NOR granularcounts could be of great referent value to dignosing pernicious degree of gas-tric tumor;it was also proved by the electron microscopy that the silverstaining substances were located in the NOR,and an energy spectrum analysison the positive silver staining region indicated the Ag peak,which showedthe specificity for the silver staining reaction.

  • 【文献出处】 中国组织化学与细胞化学杂志 ,Chinese Journal of Histochemistry and Cytochemistry , 编辑部邮箱 ,1993年01期
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