【摘要】 本文研究了用原子吸收分光光度法间接测定生物样品中游离脂肪酸（FFA）的方法,用氯仿-正己烷-甲醇混合液萃取样品中FFA,用铜溶液与FFA形成“铜皂”,进入有机相,未反应的Cu²⁺进入水相,用原子吸收法测定有机相中铜,间接求得游离脂肪酸含量。本法线性范围为10～100nmol,对血浆测定结果的相对标准偏差为<6.59％,回收率为96％～102％。本法的测定结果与比色法的结果基本一致。更多还原

【Abstract】 A new method for the determination of free fatty acid (FFA) in organism samples using atomic absorption spectrometry was studied. The FFA was extracted from subcellular organelles with chloroform-n-hexane-methanol. Copper ion was added to the organic phase to form the copper soaps with FFA. FFA was determined indirectly by determining the copper in the organic phase. The assay shows a linear response over the range of 10 to 100 nmol of FFA. The recovery of FFA from plasma is 96%-102% . The relative standard deviation is 6. 59%. The result of the determination of sample by this method is in agreement with that by colorimetric method.更多还原