【摘要】 以不同PBR322质粒DNA片段作为YAC(yeast artificial chromosome,酵母人工染色体)DNA左右臂探针,使用稀切点限制性内切酶和脉冲电泳(pul-sed field electrophoresis)技术对含有2bA3的800kbYAC作了4种限制酶大尺度物理图谱分析。更多还原

【Abstract】 Different fragments of plasmid pBR322 DNA were used as the left and right arms probes of YAC (Yeast Artificial Chromosome). The rare cutting restriction enzyme and pulsed field electrophoresis were used for long range physical mapping of YACs. A YAC containing 2bA3 DNA locus with the length of 800 kb was analysed with four different restriction enzyme to obtain the long range physical map.更多还原