【摘要】 从大庆油田油井产出油水混合物中分离到一株Microbacterium 91A。该菌株为革兰氏阳性,不形成芽孢的好氧菌。它能在72℃牛奶中存活18min,并不能还原硝酸盐。在以刃天青作为氧化还原电位指示剂的严格厌氧条件下,菌株Microbacterium 91A生长在含有0.04% Tween 80的无机盐培养基中,分别以大庆原油和正壬烷作为唯一碳源,40℃接床培养25天,用气相色谱和质谱检测到了代谢产物4-甲基-2.6-二特丁基苯酚的存在。更多还原

【Abstract】 A strain of Microbacterium 91A was isolated from the producing well of Daqing oil-field. It is a grampositive, nonspore-forming aerobe. This strain can survive in 72℃milk for 18 minutes and can’t reduce nitrate. On a reciprocal shaker (150rpm) at 40℃, under strictly anaerobial conditionswhere Hungate technology was used, the strain of Microbacterium 91A was grown on amineral medium containing 0. 04% Tween 80. Daqing oil and n-nonane were respective-ly added as the carbon sources in final concentrations of 4%. 4-methyl-2. 6-di-tert-phe-nol was detected by gas chromatograph and mass spectrum in both circumstances.更多还原