【摘要】 根据药代动力学原理,采用荧光偏振免疫法(FPIA)检测不同时间内豚鼠外淋巴中庆大霉素的浓度,了解其蓄积的情况。结果表明:(1)外淋巴内的药物浓度随庆大霉素用药量的增加而上升,基本上呈线性关系:(2)在注射7d的一组动物,停药72h仍能测到药物,其浓度接近血清最低有效浓度,达1.3±0.95μg/ml,提示内耳对药物排泄十分缓慢,确实存在外淋巴对庆大霉素的明显蓄积:(3)前述事实表明,即使已停药数日,药物的耳毒作用仍在继续,从而解释了临床上有些病人在停药后仍出现耳毒症状或症状加剧的问题。更多还原

【Abstract】 According to the pharmacokinetic theory, the authors studied the accumulation of gentamycin in perilymph of guinea pigs by searching the drug concentration at various time with Fluorescene Polarization Immunoassay (FPIA). The results indicated that: (1) The drug concentration in perilymph increased with the augment of total doses administered and there was almost a linear correlation between them. (2) In animals of 7-day injectious group, the drug could be still detected during 72 hours after the last dose administration. The concentration was 1 37±0.95 μg/ml which was close to the level of serum minimal inhibitory concentration (MIC). It suggested that the elimination of the drug from inner ear was too slow and evident accumulation of gentamycin was indeed. (3) The fact mentioned above implied that the drug ototoxities could still damage the ear even if the drug administered had ceased before. It is the reason by which we could explain the problem encountered in clinic, that is why in some patients the ototoxic syndrom may still presented or enhanced even though the treatment has been stopped a few days before.更多还原