【摘要】 用以选择犬瘟热病毒RNA的有关碱基顺序,设计DNA探针,并在5′末端偶联一个氨基连接分子（Aminolinkl）:5′×AGGGCTCA-GGTAGTCCAGCAATG3′,共23个碱基和一个氨基连接分子。NH₂-DNA探针在DNA合成仪上合成。合成产物同生物素酰氨基已酸盐N—羟琥珀酰亚胺酯（Biotinamidocaproate N-hydroxysuccinimide ester）反应,反应物经高效硅胶薄层板纯化,得到生物素标记犬瘟热病毒DNA探针。更多还原

【Abstract】 A segment of RNA base sequence was selected from Canine distemper virus for DNA probe,with the addition of an Aminolink at. the 5’ end: 5’ XAGGGCTCAGGTAGTCCAGCAATG3’, 23 bases and an aminolink. NH₂-DNA probe was synthesized on DNA synthesizer. The synthesized product reacted with biotinamidocaproate N-hydroxysuccinimide ester to biotinylate the probe. The biotynylated probe was purified by silica,gel thin layer chromatography.更多还原