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诱变与组培相结合提高小麦变异频率的研究
Variation Enhancement by Immature Embryo Culture Combined with Mutagenic Treatments in Common wheat
【摘要】 以3个普通小麦推广品种为材料,种子先经理化诱变处理后(EMS0.2M,r—射线30KR,SA2mM),接种M1代幼胚进行离体培养,比较了再生R2代不同处理的变异频率。结果表明,EMS 处理后R2代的变异频率和不分离变异所占比例最高;有益变异频率的提高以r—射线处理的R2代最为显著,SA 处理也具有提高R2变异频率的作用,但其有益变异及不分离变异与R2对照接近。诱变处理后R2代与R2对照相比,对变异谱没有明显影响。
【Abstract】 The effect of mutagenic treatments combined with immature embryoculture on the variation frequency of common wheat in R2 generation(M3R2)wastested using three wheat varieties.The immature embryo derived from theseeds pre-treated with mutagens(EMS0.2M,r-radiation 30KR,SA 2mM)was inoculated.The results showed that the M2R2 treated with EMS hadthe highest variation frequency and proportion of non-segregating variationin R2 generation.The highest useful variation frequency were found inr-radiated material.Although the variation frequency was increased in SAtreatment,the frequency of useful variation and proportion of non-segregatingvariation had no difference with control(R2).No evidence was found thatvariation spectrum was enlarged in mutagenic treated material than R2.
- 【文献出处】 河北农业大学学报 ,Journal of Agricultural University of Hebei , 编辑部邮箱 ,1993年02期
- 【被引频次】1
- 【下载频次】34