【摘要】 研究证实培养人眼视网膜色素上皮（RPE）细胞具有β₂肾上腺能受体。异丙肾上腺素、Sa-lbutamol和多巴胺均通过激活β₂受体诱导细胞内cAMP合成,其效应可被β受体拮抗剂心得安和β₂受体拮抗剂ICI 118551所阻断,而β₁受体拮抗剂CGP20712 A对之无影响;作为选择性β₂受体激动剂的Pi-ndolol和OXPrenolol也不影响cAMP的基础水平。结果提示:在人眼RPE细胞,其β肾上腺能受体以β₂受体亚型为主要存在形式;β₂受体对RPE细胞复杂的生理功能可能具有重要的调控作用。更多还原

【Abstract】 Cultured retinal pigment epithelial （RPE）cells prepared from post-mortem adult human eyes areShown to possess β₂-adrenergic receptors associated with production of the introcellular cAMP.Isopro—terenol,salbutamol and dopamine all stimulate the production of cAMP via β₂-type receptors Theireffect could be blocked by propranolol and a selective β₂-antagonist ICI 118551,but β₁-antagonistCGP 20712 A have no effect on a.Nor do the β₃-agonists pindolol and oxprenolol stimulate the cAMPproduction.It is therefore suggested that the β-adrenergic response in cultured human RPE is mainlymediated by β₂-receptors,which may have a possible physiological role in the regulation of RPE cellfunction.更多还原